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1
2019 - 01 - 07
2019年1月7日 讯 /生物谷BIOON/ --诱发癌症有很多遗传原因,比如有些突变会遗传自父母,而其它则是后天获得性的突变,比如因外界因素或DNA复制的错误导致等,大规模的基因组测序在识别因体细胞突变所诱发的癌症上取得了一系列研究成果,但这种技术却无法有效识别因遗传性突变所诱发的癌症,而且识别这些遗传突变的主要来源仍然是基于家族性的研究。图片来源:iran-daily.com近日,一项刊登在国际杂志Nature Communications上的研究报告中,来自巴塞罗那基因组研究中心的科学家们通过研究开发了一种新型的统计学方法,其能够从肿瘤测序数据中鉴别出癌症易感性基因;该方法使用了一种古老的想法,即癌基因通常需要“两次击打”(two hits)才能够致癌,研究者表示,这种方法能帮助他们从当前癌症基因组数据库中系统性地鉴别出相关的基因。研究者将这种统计学方法命名为“ALFRED”,目前该技术已经鉴别出了13个候选的癌症易感性基因,其中10个基因都是此前并未发现的;Ben Lehner博士说道,我们对30种不同类型的肿瘤患者进行研究,将这种方法应用于1万多名患者的基因组序列中,最终鉴别出了已知的和一些新型的可能性癌症易感基因,这些基因有可能会诱发一定的患癌风险。研究者指出,新型的癌症易感基因或许在多种类型癌症的发生上扮演着关键角色,比如,其与14%的卵巢肿瘤、1%的乳腺癌等癌症直接...
2
2019 - 05 - 27
5月24日,记者从中国农业科学院科研进展通报会上获悉,由中国农业科学院哈尔滨兽医研究所(以下称哈兽研)自主研发的非洲猪瘟疫苗取得了阶段性成果,实验室研究结果表明,具有良好的生物安全性和免疫保护效果。非洲猪瘟疫苗科研攻关负责人介绍,目前在非洲猪瘟疫苗创制阶段主要取得五项进展:一是分离我国第一株非洲猪瘟病毒。建立了病毒细胞分离及培养系统和动物感染模型,对其感染性、致病力和传播能力等生物学特性进行了较为系统的研究,并揭示了我国非洲猪瘟流行毒株的基因组特点和进化关系。二是创制了非洲猪瘟候选疫苗,实验室阶段研究证明其中两个候选疫苗株具有良好的生物安全性和免疫保护效果。三是两种候选疫苗株体外和体内遗传稳定性强。分别将两种候选疫苗株在体外原代细胞中连续传代,其生物学特性及基因组序列无明显改变,猪体内连续传代,也未发现明显毒力返强现象。四是明确了最小保护接种剂量,证明大剂量和重复剂量接种安全。五是临床前中试产品工艺研究初步完成。目前已建立两种候选疫苗的生产种子库,初步完成了疫苗生产种子批纯净性及外源病毒检验,初步优化了候选疫苗的细胞培养及冻干工艺。非洲猪瘟疫情发生后,党中央、国务院高度重视疫情防控科研攻关。中国农科院科技局局长任天志介绍,按照农业农村部党组统一部署,中国农科院将非洲猪瘟防控作为服务国家战略需求的重大科研攻关任务,集聚优势资源,第一时间组建非洲猪瘟防控科技攻关项目组,以哈尔滨兽医研究...
3
2019 - 01 - 21
冬季流感严重危害人类健康,甚至会威胁生命,我们究竟有什么办法预防流感呢?目前,美国密歇根大学 Betsy Foxman 和同事最新研究表明,呼吸道微生物群可能与流感易感性密切相关。这项研究报告发表在 1 月 9 日出版的《美国公共科学图书馆 · 综合》杂志上。流感病毒是流感病原体,主要侵袭目标是人体呼吸道,感染上皮细胞。鼻子和喉咙的上皮细胞被复杂的细菌群落包围,因此 Betsy Foxman 和同事推测认为,这些呼吸道微生物群可能与病毒相互作用,并在人体对流感预防方面充分发挥作用。在这项研究中,研究人员对 2012—2014 年登记的 144 个尼加拉瓜家庭进行了分析,这些家庭中有一位成员被确认患有流感,包括成年人和儿童的所有家庭成员都接受为期两个星期的监测。研究人员对每个家庭进行 5 次回访信息统计,对所有家庭成员的呼吸道微生物取样,并对监控测试者进行症状日志记录。研究者使用统计模型,对每次测试的家庭成员呼吸道微生物群分类为两个不同种群类型,尽管大约一半的测试者在研究测试过程中体内微生物群类型发生了变化。接下来,研究人员将参与者微生物类型与他们流感易感性进行了对比分析。结果发现,跨年龄组的一种呼吸道微生物类型显示出对流感的易感性降低,这种微生物类型在婴儿和儿童群体中并不常见,而且与年龄较大的青少年和成年人相比,其稳定性较低。虽然微生物种群和流感易感性之间的联系在这项研究...
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总部地址:北京市海淀区安宁庄东路23号银燕大厦D座2层201室
产品名称:

EVOS™ FL Imaging System

上市日期: 2019-04-15

规格

Color:Transmitted Light
Data Output:BMP Files, JPG Files, PNG Files, TIFF Files
Detection Method:Fluorescent
Dimensions:36 cm (14 in) (W) x 58 cm (23 in) (H) x 47 cm (19 in) (D)
Display Interface:15-inch color monitor, Adjustable tilt
For Use With (Equipment):Fluorescence Microscope
Format:Specific Holder Attachments
Frequency:50⁄60 Hz
Input Voltage:100-240 VAC
Objectives:5 Objective Turret
Product Line:EVOS®
Product Size:1 each
Stage:Mechanical stage with X-Y axis fine-positioning controls
Weight:15.3 kg (33.7 lbs)


产品类别: EVOS
  • 产品描述
  • 产品功能
  • 产品参数

描述

The EVOS® FL Imaging System is a fully integrated, digital, inverted imaging system for four-color fluorescence and transmitted-light applications. It is powerful, yet easy-to-use and delivers high-definition images with exceptional convenience. The unique light cubes, sensitive camera, and precision-engineered optical system make the EVOS® FL system ideal for both demanding and routine fluorescence imaging applications.

The EVOS® FL Imaging System offers you these important advantages:

• Easy installation; no maintenance, assembly, alignment, or calibration
• For four-color fluorescence and transmitted light applications
• Five-position objective turret with front controls
• On-board software
• All-in-one design: digital camera, precision optics, LCD display, and USB storage

Fully Integrated Imaging System
The EVOS® FL system represents a new concept in transmitted light inverted microscopy. It is a fully integrated imaging system that combines precision optics, a 15' high-resolution LCD display, and a highly sensitive Sony ICX445 monochrome CCD camera (1280 x 960 pixel resolution, 1.3 Megapixels). Images are seamlessly acquired through the intuitive user interface using a mouse for easy control. Monochrome cameras are commonly used for high-performance fluorescence applications and provide the best sensitivity for detection of faint fluorescence signals. Color cameras, as on the EVOS® FL Color System, have lower fluorescence sensitivity but have the advantage of being able to differentiate structures by color in transmitted light (e.g., imaging stained tissue samples).

Compare cell imaging systems >

Versatile and Highly Configurable
The EVOS® FL system features an easily accessible 5-position objective turret and a 3-position condenser turret. The 4 fluorescence light cubes are lever-actioned for rapid and easy selection. The optical system can be configured to best meet your needs using our full range of high-quality objectives from 2x to 100x magnification. Lighting settings are automatically adjusted to match the objective magnification. The EVOS® FL system features a mechanical stage with X/Y-axis fine-positioning using control knobs conveniently located on the left side and the front of the stage. The stage accommodates EVOS® vessel holders that provide a perfect fit for most vessel types and sizes. Interchangeable stage plates accommodate most vessel types and sizes, including flasks, Petri dishes, multi-well plates, and slides.

Smart LED Illumination Technology
All EVOS® fluorescence imaging systems utilize our unique, proprietary LED light cubes. This world-leading light engine outputs remarkable intensity over a short light-path that delivers superior fluorophore excitation. Each light cube contains a precisely matched set of optical components to optimize the position, evenness, and intensity of the light beam. Digitally controlled LED light sources allow adjustment of illumination levels, dramatically improving control over photobleaching. Hard-coated filters give sharper edges and significantly higher transmission efficiencies than traditional soft-coated filters.

Integrated Software
The on-board computer makes the EVOS® FL system easy to run and use, and the intuitive, comprehensive software facilitates both image acquisition and analysis. Modules include time-lapse acquisition, manual-assist cell counting, and image review. Saved user settings work in conjunction with 'smart' controls to help increase efficiency and ease of use. Image data are saved to a USB flash drive for easy, portable data transfer or to a Windows-networked local server. Images can be saved in PNG, JPEG, BMP, and TIFF formats with a maximum captured image resolution of 1280 x 960 pixels.

These features make the EVOS® FL system ideal for both routine and demanding fluorescence imaging applications such as GFP-vector transfection efficiency analysis, routine cell and tissue culture, cell confluence determination, stem cell growth and differentiation, and developmental biology and tissue slice analyses. And the compact footprint makes it as easy to use the EVOS® FL system in a cell culture hood as on the bench.

Easy to Use and Reliable
The EVOS® FL Imaging System is plug-and-play. In addition to an exceptionally easy set-up and installation, it requires no warm-up or cool-down periods. The LED light source offers exceptional stability and durability—so you can turn the unit on and off whenever you need to image a sample. The environmentally safe mercury-free LED bulbs are rated for >50,000 hours (~17 years) of life, compared to 300 hours for a typical mercury bulb and 1,500 hours for a metal halide bulb. The long life and low energy consumption translate into significantly lower operating costs compared to instruments with conventional light sources. Finally, the advanced ergonomic design of the EVOS® systems removes the strain associated with conventional microscopes, enables shared viewing, and makes moving the units easy. For extended or precision use, the EVOS® arm rest provides comfortable support.

The EVOS® imaging systems are built from the ground up to maximize performance and optimize workflow. You will be astonished at how easy it is to operate and amazed at how extraordinary your images look on-screen.

Learn more about the EVOS® FL Imaging System
Explore the entire EVOS® line of imaging systems and accessories

System Highlights
Optics: infinity-corrected optical system, RMS-threaded objectives with 45 mm parfocal distance
Illumination: adjustable intensity LED (>50,000-hour life per light cube)
Light cubes (not included): broad selection of patented standard and specialty light cubes
Common light cubes include: DAPI (Ex 360 nm/Em 447 nm), GFP (Ex 470 nm/Em 525 nm), RFP (Ex 530 nm/Em 593 nm), Texas Red® (Ex 585 nm/Em 624), Cy5 (Ex 628 nm/Em 692 nm)
Contrast methods: fluorescence and transmitted light (brightfield & phase contrast)
Objective turret: 5-position, front-mounted control
Objectives (not included): wide selection of high-quality LWD and coverslip-corrected objectives available
Condenser: 3-position turret with brightfield and phase contrast annuli
Condenser working distance: 60 mm
Stage: mechanical 'glide' stage; X-Y axis fine-positioning controls: 28.3 mm (1.11') per rotation, 110 mm x 110 mm (4.3' x 4.3') range of motion; Z-axis focusing controls: 480 µm/rotation; interchangeable vessel holders are available for most common shapes and sizes
Focus mechanism: coaxial focus knobs with tension control
Course focus: 38 mm/rev
Fine focus: 0.2mm/rev, precision 0.002 mm
LCD display: 15-inch color, 1024 x 768 pixels, adjustable tilt
Camera: Sony ICX445 monochrome CCD, 1/3' 1280 x 960 pixels, 1.3 Megapixels
Image acquisition: onboard microprocessor, built-in software for image acquisition via mouse control
Captured images: 16-bit monochrome TIFF or PNG (12-bit dynamic range); 24-bit color TIFF or PNG; jpeg, bmp, 1280 x 960 pixels
Output ports: 3 USB and 1 DVI
Power supply: AC adapter with country-specific power cords; input 100-240 V, 50-60 Hz; output 12 VDC/4.15 A
Dimensions: operating height = 57.8 cm (22.75 in); storage/transport height = 32.4 cm (12.75 in); depth = 47.0 cm (18.5 in); width = 35.5 cm (14.0 in)
Weight: 15.3 kg (33.7 lbs) 


规格

Color:Transmitted Light
Data Output:BMP Files, JPG Files, PNG Files, TIFF Files
Detection Method:Fluorescent
Dimensions:36 cm (14 in) (W) x 58 cm (23 in) (H) x 47 cm (19 in) (D)
Display Interface:15-inch color monitor, Adjustable tilt
For Use With (Equipment):Fluorescence Microscope
Format:Specific Holder Attachments
Frequency:50⁄60 Hz
Input Voltage:100-240 VAC
Objectives:5 Objective Turret
Product Line:EVOS®
Product Size:1 each
Stage:Mechanical stage with X-Y axis fine-positioning controls
Weight:15.3 kg (33.7 lbs)


规格

Color:Transmitted Light
Data Output:BMP Files, JPG Files, PNG Files, TIFF Files
Detection Method:Fluorescent
Dimensions:36 cm (14 in) (W) x 58 cm (23 in) (H) x 47 cm (19 in) (D)
Display Interface:15-inch color monitor, Adjustable tilt
For Use With (Equipment):Fluorescence Microscope
Format:Specific Holder Attachments
Frequency:50⁄60 Hz
Input Voltage:100-240 VAC
Objectives:5 Objective Turret
Product Line:EVOS®
Product Size:1 each
Stage:Mechanical stage with X-Y axis fine-positioning controls
Weight:15.3 kg (33.7 lbs)


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